Journal: International journal of molecular sciences

Article Title: α3β4 Acetylcholine Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells.

doi: 10.3390/ijms23169101

Figure Lengend Snippet: Figure 3. Membrane colocalization and overlapping between α3β4 nAChR (red) and DBH (green) after secretion stimulus: in each image we individually selected several regions of interest (ROIs) (a,b) and proceeded to analyze them using ImageJ JACoP complement (See Section 4.6), obtaining the values of the Pearson’s and Manders coefficients, whose averages are shown in Table 1, as well as their corresponding scatterplot graphs, whose examples are shown (d). The averaged Pearson’s coeficients for 50% threshold images show a significant colocalization between endogenous α3β4 nAChRs structures and the DBH sites in both stimulation conditions to those obtained for randomly simulated α3β4 nAChRs structures (Table 1 (c)). Furthermore, the averaged coefficients obtained for the stimulus with ACh are significantly higher than those obtained when stimulating with a high K+ (p value *** < 0.0001; Table 1). (d) A scatterplot or fluorogram for pixel colocalization of the red channel (AChR structures) and green channel (DBH) comparing three examples of the images obtained in each of the three analyzed conditions. Bars represent 1 µm.

Article Snippet: We revealed the immunological location of the endogenous β4 subunits using a rabbit polyclonal antibody anti-Nicotinic Acetylcholine Receptor β4 (extracellular)-Atto-594 (alomone Labs, Cat #: ANC-014-AR; Lot: ANC014ARAN0150, Jerusalem, Israel).

Techniques: Membrane